Who Should Attend?  

 

Researchers and professionals interested in

• Regulation of metabolic machinery

• Inborn errors of metabolism

• Metabolic reprogramming in diseases and disorders

• Metabolic response to environmental exposure

• Nutrition

• Drug metabolism & Toxicology

• Identification of biomarkers

• Assay development for clinical biochemistry

• Identification of novel metabolites and pathways

 

N.B. Attending the school and the symposium is a prerequisite to participate in the workshop since fundamentals of mass spectrometry will be covered there. Only for up to four participants, this requirement will be waived provided they have significant expertise in mass spectrometry and chromatography.

     

 

 

What will be covered?

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Principles, practical considerations and hands-on sessions on-

• Extraction of metabolites from various biofluids, tissues, and cells

• Development of chromatographic and mass spectrometric methods

• Quality control samples

• Sources of error

• Data deconvolution and feature extraction

• Statistical (univariate and multivariate) analysis

• Metabolite identification

• Comparative analysis of metabolite composition of biological matrices

• Bioinformatic analysis

 

 

Instructor (Theory)

 

Prof. Philip Britz-McKibbin (McMaster University, Canada)

Dr. Augustin Scalbert (IARC-WHO, France)

Dr. Daniel Globisch (Uppsala University, Sweden)

Dr. Soumen Kanti Manna (Saha Institute of Nuclear Physics, India)

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Instructor (Hands-on)

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Dr. Soumen Kanti Manna (SINP, India)

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Demonstrator (Hands-on)

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Mr. Sk Ramiz Islam (SINP, India)

Saran Chattyopadhyay (SINP, India)

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Workshop Programme schedule

 

 

Dates: November 15-17, 2019

Venue: Biophysics & Structural Genomics Division, SINP, Kolkata

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November 15 (Friday)

 

10:00 – 13:00      Principles and practical considerations for method development  Principles and practical                                      considerations for metabolite extraction and sample preparation for metabolic profiling

                            (S K Manna)

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                            Principles and practical considerations for analysis of metabolomic signatures to identify                                      biomarkers

                            (Augustin Scalbert)

 

13:00 – 14:00                                                                     LUNCH BREAK

 

14:00 – 16:30    Pattern Recognition & Metabolite Identification Multiplexed CE-MS Technology for                                                Metabolomics and Biomarker Discovery: Doing More With Less

                          (Philip Britz-McKibbin)

 

                          Identification of metabolites

                          (Daniel Globisch)

 

16:30 – 19:00     Metabolite extraction (hands-on)

                           Preparation of solvents, standard mixtures, quality control samples

                           Extraction of metabolites using different solvents and solvent mixtures from cell, tissue,                                         plasma/serum, urine, saliva.  

 

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November 16 (Saturday)

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10:00 – 13:30    Method development (hands-on)

                          Sample preparation for MS

                          Designing chromatographic methods

                          Designing MS method

                          Loading samples for analysis

 

13:30 – 15:00                                                                            LUNCH BREAK

 

15:00 – 15:30    Quiz

 

15:30 – 19:00    Data Analysis (hands-on)

                          Observing instrument performance

                          Data deconvolution, feature extraction

                          Analyzing quality control samples

                          Metabolite identification

 

 

 

November 17 (Sunday)

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10:00 – 13:00    Comparative Analysis (hands-on)

                          Comparative analysis of the effect of extraction method on metabolic signature

                          Analysis of the effect of chromatographic and mass spectrometric methods on metabolic                                      signature

 

13:00 – 14:30                                                                        LUNCH BREAK

 

14:30 – 15:30    Comparative Analysis (hands-on)

                          Comparative analysis of metabolite composition of biological matrices

 

16:00 – 18:30    Statistical treatment and Bioinformatic analysis (hands-on)

 

18:00 – 19:00    Quiz and discussion

 

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